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(a) Western Blot of IGF1R alpha after SNA pulldown in <t>ST6GAL1</t> knockout cells (b) Western blot of IGF1R alpha immunoprecipitated with ganitumab in ST6GAL1 knockout cells (c) Western blot of p-IGF1R in ST6GAL1 knockout cells treated with ganitumab (d) ST6GAL1 expression in ovarian cancer tumor vs normal ovary tissue from TCGA data analyzed by Xenabrowser. (e) Ganitumab H-score of ovarian cancer FFPE tissues stratified into ST6GAL1-High (n=12) and ST6GAL1-Low (n=12) after sialidase (SIA) digestion; representative image of the staining and H&E staining of the tissues shown at 40x magnification. Scale bar = 50μm.
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Image Search Results


(a) Western Blot of IGF1R alpha after SNA pulldown in ST6GAL1 knockout cells (b) Western blot of IGF1R alpha immunoprecipitated with ganitumab in ST6GAL1 knockout cells (c) Western blot of p-IGF1R in ST6GAL1 knockout cells treated with ganitumab (d) ST6GAL1 expression in ovarian cancer tumor vs normal ovary tissue from TCGA data analyzed by Xenabrowser. (e) Ganitumab H-score of ovarian cancer FFPE tissues stratified into ST6GAL1-High (n=12) and ST6GAL1-Low (n=12) after sialidase (SIA) digestion; representative image of the staining and H&E staining of the tissues shown at 40x magnification. Scale bar = 50μm.

Journal: bioRxiv

Article Title: Cancer-specific sialylation of insulin-like growth factor 1 receptor impairs therapeutic antibody binding and efficacy

doi: 10.1101/2025.10.17.682592

Figure Lengend Snippet: (a) Western Blot of IGF1R alpha after SNA pulldown in ST6GAL1 knockout cells (b) Western blot of IGF1R alpha immunoprecipitated with ganitumab in ST6GAL1 knockout cells (c) Western blot of p-IGF1R in ST6GAL1 knockout cells treated with ganitumab (d) ST6GAL1 expression in ovarian cancer tumor vs normal ovary tissue from TCGA data analyzed by Xenabrowser. (e) Ganitumab H-score of ovarian cancer FFPE tissues stratified into ST6GAL1-High (n=12) and ST6GAL1-Low (n=12) after sialidase (SIA) digestion; representative image of the staining and H&E staining of the tissues shown at 40x magnification. Scale bar = 50μm.

Article Snippet: The antibodies and lectin used include: IGF1R α (Santa Cruz, Catalog# sc-271606), IGF1R β (Cell Signaling Technology, Catalog# 3027), phospho-IGF1R β (Cell Signaling Technology, Catalog# 4568), ST6GAL1 (R&D Systems, Catalog# AF594), β-actin (Thermo Fisher, Catalog# MA1-140), and Sambucus Nigra (SNA) lectin (Vector laboratories, Catalog# B-1305-2).

Techniques: Western Blot, Knock-Out, Immunoprecipitation, Expressing, Staining